描述

膽固醇-PEG-NHS酯與胺反應(yīng)形成穩(wěn)定的酰胺鍵用于生物偶聯(lián)。膽固醇是細(xì)胞膜成分，具有調(diào)節(jié)膜流動(dòng)性、彈性和滲透性的功能。當(dāng)?shù)鞍踪|(zhì)被包埋在膜中時(shí)，它可填補(bǔ)由其他脂質(zhì)種類的包裝產(chǎn)生的間隙。聚乙二醇化膽固醇作為一種兩親性脂質(zhì)衍生物，可用于制備脂質(zhì)體和其他膠束作為藥物傳遞系統(tǒng)。諸如琥珀酰亞胺基NHS酯的官能團(tuán)將在膠束或脂質(zhì)體表面上結(jié)合包括蛋白質(zhì)、抗體和染料在內(nèi)的含胺部分。

Properties

Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).

Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.

Density: PEG density is approximately 1.125 g/mL

Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.

Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.

References

1.. Polymer stealthing and mucin-1 retargeting for enhanced pharmacokinetics of an oncolytic vaccinia virus, Molecular Therapy: Oncolytics, Vol. 21 June 2021, Text.

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